Drosha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03349
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1324 and 1374 of human double-stranded RNA-specific endoribonuclease using the numbering given in entry NP_037367.3 (GeneID 29102).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25351346).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Drosha Antibody
![Western Blot: Drosha Antibody [NBP1-03349]](https://resources.bio-techne.com/images/products/Drosha-Antibody-Western-Blot-NBP1-03349-img0006.jpg "Western Blot: Drosha Antibody [NBP1-03349]")
Western Blot: Drosha Antibody [NBP1-03349]
Western Blot: Drosha Antibody [NBP1-03349] - Detection of Human Drosha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunoprecipitation: Drosha Antibody [NBP1-03349]](https://resources.bio-techne.com/images/products/Drosha-Antibody-Immunoprecipitation-NBP1-03349-img0007.jpg "Immunoprecipitation: Drosha Antibody [NBP1-03349]")
Immunoprecipitation: Drosha Antibody [NBP1-03349]
Immunoprecipitation: Drosha Antibody [NBP1-03349] - Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for IP at 6 ug per reaction. Drosha was also immunoprecipitated by another rabbit anti-Drosha antibody. For blotting immunoprecipitated Drosha, NBP1-03349 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for Drosha Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID 25351346)
Immunohistochemistry
1:250
Immunohistochemistry-Frozen
Reported in scientific literature (PMID 25351346)
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Reviewed Applications
Read 3 reviews rated 4 using NBP1-03349 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Drosha
Alternate Names
drosha, double-stranded RNA-specific endoribonuclease, drosha, ribonuclease type III, EC 3.1.26.3, ETOHI2, nuclear RNase III Drosha, p241, Protein Drosha, putative protein p241 which interacts with transcription factor Sp1, putative ribonuclease III, RANSE3L, ribonuclease 3, Ribonuclease III, ribonuclease III, nuclear, RN3nuclear, RNase III
Entrez Gene IDs
29102 (Human)
Gene Symbol
DROSHA
UniProt
Additional Drosha Products
Product Documents for Drosha Antibody
Product Specific Notices for Drosha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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