Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all DRP1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein. [Swiss-Prot# O00429]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34622072). Fish reactivity reported in scientific literature (PMID: 25008790).

Localization

Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DRP1 Antibody - BSA Free

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-DRP1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: DRP1 Antibody - BSA Free [NB110-55288]

Immunohistochemistry-Paraffin: DRP1 Antibody [NB110-55288] - Analysis of FFPE tissue section of mouse kidney using DRP1 antibody #NB110-55288 at 1:300. The primary antibody bound to DRP1 protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated a diffused cytoplasmic staining of DRP1 in the epithelial cells of various tubules and in the cells of glomeruli.

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout. Lane 2: DRP1 wildtype MEFs. Stained with NB110-55288 at 1:500.

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: DRP1 Antibody - BSA Free [NB110-55288]

Immunohistochemistry: DRP1 Antibody [NB110-55288] - Staining of renal tubular epithelium and visceral epithelial cells of the glomerulus. Human kidney cortex, 40X magnification.

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody [NB110-55288] - Assessment of skeletal muscle mitochondrial dynamic markers. Representative western blots. Significant main effect of training: #, p < 0.05; ##, p < 0.01. Significant post-hoc training effect: *, p < 0.05; **, p < 0.01. Data are presented as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: DRP1 Antibody - BSA Free [NB110-55288]

Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with DRP1 Antibody NB110-55288AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody [NB110-55288] - Rat spinal cord-DRP1 (81kda). Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5.0 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse Dylight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: DRP1 Antibody - BSA Free [NB110-55288]

Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

Simple Western: DRP1 AntibodyBSA Free [NB110-55288]

Simple Western: DRP1 Antibody [NB110-55288] - Image shows a specific band for DUX4 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: DRP1 AntibodyBSA Free [NB110-55288]

Simple Western: DRP1 Antibody - BSA Free [NB110-55288] - Image shows a specific band for DRP1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.

Western Blot: DRP1 Antibody - BSA Free [NB110-55288] -

Assessment of skeletal muscle mitochondrial dynamic markers. (A) Total Drp1 protein content, (B) phospho-Drp1Ser616 protein content, (C) percent difference in total and phospho-Drp1Ser616 in ET participants relative to SED participants, (D) total Mfn1 protein content, (E) total Mfn2 protein content. (F) Representative western blots. Significant main effect of training: #, p < 0.05; ##, p < 0.01. Significant post-hoc training effect: *, p < 0.05; **, p < 0.01. Data are presented as mean ± SEM.

View 12 more images

Applications for DRP1 Antibody - BSA Free

Application

Recommended Usage

Flow (Intracellular)

1.0 ug/ml

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Immunoprecipitation

reported in scientific literature

Simple Western

1:50

Western Blot

1:500

Application Notes

In Western blot a band is seen at ~81 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using NB110-55288 in the following applications:

Simple Western (1 Review)
Western Blot (3 Reviews)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DRP1

DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).

Long Name

Dynamin-1-like protein

Alternate Names

DLP1, DNM1L, DVLP, Dymple, HdynIV

Entrez Gene IDs

10059 (Human); 74006 (Mouse); 114114 (Rat)

Gene Symbol

DNM1L

UniProt

O00429 (Human)

Additional DRP1 Products

Product Documents for DRP1 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download IHC-P Protocol

Download Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for DRP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

DRP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55288

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all DRP1 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for DRP1 Antibody - BSA Free

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Immunohistochemistry-Paraffin: DRP1 Antibody - BSA Free [NB110-55288]

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Immunohistochemistry: DRP1 Antibody - BSA Free [NB110-55288]

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Flow Cytometry: DRP1 Antibody - BSA Free [NB110-55288]

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody - BSA Free [NB110-55288]

Flow Cytometry: DRP1 Antibody - BSA Free [NB110-55288]

Simple Western: DRP1 AntibodyBSA Free [NB110-55288]

Simple Western: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody - BSA Free [NB110-55288] -

Applications for DRP1 Antibody - BSA Free

Flow (Intracellular)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Simple Western

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: DRP1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional DRP1 Products

Product Documents for DRP1 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for DRP1 Antibody - BSA Free