DSCAM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41390

Novus Biologicals, part of Bio-Techne
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NB100-41390
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DSCAM Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DSWDSAQRTKDVSPQ corresponding to internal region according to NP_996770.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_001380.2 and NP_996770.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DSCAM Antibody

Western Blot: DSCAM Antibody [NB100-41390]

Western Blot: DSCAM Antibody [NB100-41390]

Western Blot: DSCAM Antibody [NB100-41390] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: DSCAM Antibody [NB100-41390]

Immunocytochemistry/ Immunofluorescence: DSCAM Antibody [NB100-41390]

Immunocytochemistry/Immunofluorescence: DSCAM Antibody [NB100-41390] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: DSCAM Antibody [NB100-41390]

Flow Cytometry: DSCAM Antibody [NB100-41390]

Flow Cytometry: DSCAM Antibody [NB100-41390] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for DSCAM Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

2 - 3 ug/mL
Application Notes
WB: Approx. 170 kDa band observed in human brain (cerebellum) lysates (calculated MW of 174 kDa band according to NP_996770.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DSCAM

The Dscam gene encodes a large family of axon guidance receptors and immune receptors via alternative splicing, generating over 38000 isoforms. The gene has been shown to be required for axon guidance, specifically targeting individual neurons to their targets. The gene has also been shown to be involved in the immune system of the fly; different isoforms interact with different pathogens to elicit an immune response.

Long Name

Down Syndrome Cell Adhesion Molecule

Alternate Names

CHD2-42, CHD2-52

Entrez Gene IDs

1826 (Human); 13508 (Mouse); 171119 (Rat)

Gene Symbol

DSCAM

UniProt

NP_001380.2 (Human)

Additional DSCAM Products

Product Documents for DSCAM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DSCAM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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