DUOX2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-61576

Novus Biologicals, part of Bio-Techne
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NB110-61576SS
NB110-61576
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all DUOX2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human DUOX2 (within residues 400-500). [Swiss-Prot# Q9NRD8]

Localization

Apical cell membrane; Multi-pass membrane protein. Note: Localizes to the apical membrane of epithelial cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DUOX2 Antibody - BSA Free

Knockdown Validated: DUOX2 Antibody - BSA Free [NB110-61576]

Knockdown Validated: DUOX2 Antibody - BSA Free [NB110-61576]

Knockdown Validated: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.
Western Blot: DUOX2 AntibodyBSA Free [NB110-61576]

Western Blot: DUOX2 AntibodyBSA Free [NB110-61576]

Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.
Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody - BSA Free [NB110-61576]

Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody - BSA Free [NB110-61576]

Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Applications for DUOX2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24492313)

Western Blot

2 ug/ml
Application Notes
In Western blot a band is observed at ~150 kDa, and in Immunocytochemistry/Immunofluorescence membrane staining was seen in A431 cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DUOX2

DUOX2 (dual oxidase 2), a multi-pass membrane protein localized to the apical membrane of epithelial cells, belongs to NADPH oxidase (NOX) family which is known to reduce molecular oxygen with NADPH as electron donor and unlike most of NOX-family members that produce superoxide anions (O2.-) as primary product, DUOX directly produce H2O2. DUOX2 and DUOX 1 require specific maturation factors, DUOXA1 and DUOXA2, to be functionally expressed at the apical follicular membrane, the site for hormonogenesis. DUOX2/DUOX 1 are major components of H2O2-generating system and generate H2O2 which is required for activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. DUOX2 plays a role in thyroid hormones synthesis and LPO-mediated antimicrobial defense mucosal surface. It has its own peroxidase activity through its N-terminal peroxidase-like domain which is inhibited by aminobenzohydrazide and the NADPH oxidase activity is calcium-dependent. DUOX2 is expressed in thyrocytes, intestine, kidney, liver, lung, pancreas, prostate, salivary glands, testis etc. and is induced by forskolin, thyrotropin and the Th1-specific cytokine IFNG/IFN-gamma. Defects in DUOX2 have been linked to thyroid dyshormonogenesis 6 (TDH6) characterized by defective conversion of accumulated iodide to organically bound iodine.

Alternate Names

dual oxidase 2, dual oxidase-like domains 2, EC 1.11.1.-, EC 1.6.3.1, flavoprotein NADPH oxidase, Large NOX 2, LNOX2P138(TOX), Long NOX 2, NADH/NADPH thyroid oxidase p138-tox, NADPH oxidase/peroxidase DUOX2, NADPH thyroid oxidase 2, nicotinamide adenine dinucleotide phosphate oxidase, NOXEF2, p138 thyroid oxidase, P138-TOX, THOX2TDH6, Thyroid oxidase 2

Gene Symbol

DUOX2

Additional DUOX2 Products

Product Documents for DUOX2 Antibody - BSA Free

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Product Specific Notices for DUOX2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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