Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Epitope Tag

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # F-tag-01

Format

BSA Free

Concentration

1.0 mg/ml

View all DYKDDDDK Epitope Tag Antibodies »

Product Specifications

Immunogen

DYKDDDDK Epitope Tag Antibody (F-tag-01) was made to a Synthetic peptide: DYKDDDDK conjugated to KLH.

Reactivity Notes

Tag specific

Specificity

DYKDDDDK Epitope Tag Antibody (F-tag-01) recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

1.01 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free

Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free [NB500-554]

Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free [NB500-554]

Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).
Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free [NB500-554]

Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free [NB500-554]

Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free [NB500-554]

Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free [NB500-554]

Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554]. Merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue).

Applications for DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DYKDDDDK Epitope Tag

Fusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression.

The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).
/>References
/>1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796

2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294

3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954

FLAG(R) is a trademark of Sigma-Aldrich.

Alternate Names

FLAG Tag

Additional DYKDDDDK Epitope Tag Products

Product Documents for DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DYKDDDDK Epitope Tag Antibody (F-tag-01) - BSA Free

FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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