Dynactin Subunit 1/DCTN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1110

Novus Biologicals, part of Bio-Techne
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NB100-1110
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Key Product Details

Species Reactivity

Validated:

Human, Rabbit

Cited:

Rabbit

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Proximity Ligation Assay, Western Blot

Cited:

Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Dynactin Subunit 1/DCTN1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 19136602).

Localization

Cytoplasmic

Specificity

This antibody is expected to recognise all reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dynactin Subunit 1/DCTN1 Antibody

Dynactin Subunit 1/DCTN1 Antibody

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] -

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Dynactin Subunit 1/DCTN1 Antibody

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] -

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (1ug/ml) staining of HeLa (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Dynactin Subunit 1/DCTN1 Antibody

Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] -

Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Dynactin Subunit 1/DCTN1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

6-7 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx. 150kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142kDa according to NP_004073.2). Primary incubation 1 hour at room temperature.
Use in Proximity Ligation Assay reported in scientific literature (PMID: 30354839).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dynactin Subunit 1/DCTN1

The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Alternate Names

DAP-150, DCTN1, DP-150, Dynactin 1, HMN7B

Entrez Gene IDs

1639 (Human); 13191 (Mouse)

Gene Symbol

DCTN1

UniProt

Q14203 (Human)

Additional Dynactin Subunit 1/DCTN1 Products

Product Documents for Dynactin Subunit 1/DCTN1 Antibody

Product Datasheets

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Product Specific Notices for Dynactin Subunit 1/DCTN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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