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Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7)

Novus Biologicals, part of Bio-Techne | Catalog # H00010540-M01

Novus Biologicals, part of Bio-Techne
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H00010540-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 2G7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DCTN2 (AAH00718, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Reactivity Notes

Human. Other species not tested.

Specificity

DCTN2 - dynactin 2 (p50)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Scientific Data Images for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7)

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01]

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01] - DCTN2 monoclonal antibody (M01), clone 2G7 Analysis of DCTN2 expression in MCF-7.
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01]

Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01]

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01] - Analysis of monoclonal antibody to DCTN2 on MCF-7 cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7) [H00010540-M01] - Detection limit for recombinant GST tagged DCTN2 is approximately 0.3ng/ml as a capture antibody.

Applications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dynactin Subunit 2/DCTN2

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Alternate Names

DCTN2, Dynamitin, RBP50

Entrez Gene IDs

10540 (Human)

Gene Symbol

DCTN2

OMIM

607376 (Human)

Additional Dynactin Subunit 2/DCTN2 Products

Product Documents for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (2G7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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