Dynamin 2 Antibody

Western Blot: Dynamin 2 Antibody [NB300-617]

Western Blot: Dynamin 2 Antibody [NB300-617] - Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti-Dynamin II Rabbit polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) )Secondary Antibody, HRP conjugate. A band ~98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line.

Immunocytochemistry/ Immunofluorescence: Dynamin 2 Antibody [NB300-617]

Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Trito(TM) X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, Alexa Fluor(R) 488 conjugate, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong(TM) Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing Cytoskeletal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background.

Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin II was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin II Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin . Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.