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Dynamin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3458

Novus Biologicals, part of Bio-Techne
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NB120-3458
NB120-3458-0.025ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic Peptide: C P(623) D K S F T E N D E N G Q A E N F(639)

Specificity

Detects a band at approximately 100 kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dynamin 3 Antibody

Western Blot: Dynamin 3 Antibody [NB120-3458]

Western Blot: Dynamin 3 Antibody [NB120-3458]

Western Blot: Dynamin 3 Antibody [NB120-3458] - Analysis of rat liver extract.
Immunocytochemistry/ Immunofluorescence: Dynamin 3 Antibody [NB120-3458]

Immunocytochemistry/ Immunofluorescence: Dynamin 3 Antibody [NB120-3458]

Immunocytochemistry/Immunofluorescence: Dynamin 3 Antibody [NB120-3458] - Immunofluorescence analysis of Dynamin III was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin III Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Applications for Dynamin 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles. After opening store under sterile conditions.

Background: Dynamin 3

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Alternate Names

Dyna III, dynamin 3, dynamin family member, dynamin-3, EC 3.6.5.5, MGC70433, T-dynamin, testicular

Entrez Gene IDs

26052 (Human); 171574 (Rat)

Gene Symbol

DNM3

Additional Dynamin 3 Products

Product Documents for Dynamin 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dynamin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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