Dynamin 3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB120-3458
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic Peptide: C P(623) D K S F T E N D E N G Q A E N F(639)
Specificity
Detects a band at approximately 100 kDa.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Dynamin 3 Antibody
Western Blot: Dynamin 3 Antibody [NB120-3458]
Western Blot: Dynamin 3 Antibody [NB120-3458] - Analysis of rat liver extract.Immunocytochemistry/ Immunofluorescence: Dynamin 3 Antibody [NB120-3458]
Immunocytochemistry/Immunofluorescence: Dynamin 3 Antibody [NB120-3458] - Immunofluorescence analysis of Dynamin III was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin III Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.Applications for Dynamin 3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles. After opening store under sterile conditions.
Background: Dynamin 3
Alternate Names
Dyna III, dynamin 3, dynamin family member, dynamin-3, EC 3.6.5.5, MGC70433, T-dynamin, testicular
Gene Symbol
DNM3
Additional Dynamin 3 Products
Product Documents for Dynamin 3 Antibody
Product Specific Notices for Dynamin 3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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