Dynamin Antibody (A1B2-R)

Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283]

Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: SH-SY5Y cell lysate (20 ug/Lane)
Lane 3: NIH/3T3 cell lysate (20 ug/Lane)
Lane 4: Human brain tissue lysate (40 ug/Lane)

Predicted band size: 97 kDa
Observed band size: 100 kDa

Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Dynamin Antibody (A1B2-R) [NBP3-32283]

Immunohistochemistry: Dynamin Antibody (A1B2-R) [NBP3-32283] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.