Dynamin [p Ser774] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-209
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH. Accession # P21575
Modification
p Ser774
Localization
Cytoplasmic
Specificity
Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Dynamin [p Ser774] Antibody
![Western Blot: Dynamin [p Ser774] Antibody [NB300-209]](https://resources.bio-techne.com/images/products/Dynamin-[p-Ser774]-Antibody-Western-Blot-NB300-209-img0002.jpg "Western Blot: Dynamin [p Ser774] Antibody [NB300-209]")
Western Blot: Dynamin [p Ser774] Antibody [NB300-209]
Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.Applications for Dynamin [p Ser774] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000
Application Notes
NB300-209 can be used in Western blot where a band is seen at ~ 97 kD, representing Dynamin
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Dynamin
Alternate Names
DNM1
Gene Symbol
DNM1
UniProt
Additional Dynamin Products
Product Documents for Dynamin [p Ser774] Antibody
Product Specific Notices for Dynamin [p Ser774] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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