E-Cadherin Antibody (056)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89338

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 056

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human E-Cadherin (Accession#: NP_004351.1; Met1-Ile707).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for E-Cadherin Antibody (056)

Western Blot: E-Cadherin Antibody (056) [NBP2-89338]

Western Blot: E-Cadherin Antibody (056) [NBP2-89338]

Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa
Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338]

Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338]

Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDa

Applications for E-Cadherin Antibody (056)

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (056)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for E-Cadherin Antibody (056)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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