E-Cadherin Antibody (4A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53229

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4A2

Concentration

0.2 mg/ml

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

Recombinant human E-Cadherin protein (Uniprot: P12830 )

Localization

Cell Surface

Marker

Intercellular Junction Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54587)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for E-Cadherin Antibody (4A2)

Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229]

Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229]

Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229] - Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.
Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229]

Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229]

Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229] - Staining of human pancreatic tumor. Image from a verified customer review.
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Applications for E-Cadherin Antibody (4A2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

4 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (4A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (4A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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