E-Cadherin Antibody (67A4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42793

Clone 67A4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-42793
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 67A4

Format

BSA Free

Concentration

1.0 mg/ml

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

T-47D cells

Specificity

The mouse monoclonal antibody 67A4 recognizes CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms. HLDA VIII

Marker

Epithelial Cell Marker, Adherens Junctions Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for E-Cadherin Antibody (67A4) - BSA Free

Flow Cytometry: E-Cadherin Antibody (67A4) - BSA Free [NBP1-42793]

Flow Cytometry: E-Cadherin Antibody (67A4) - BSA Free [NBP1-42793]

Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: E-Cadherin Antibody (67A4) - BSA Free [NBP1-42793]

Flow Cytometry: E-Cadherin Antibody (67A4) - BSA Free [NBP1-42793]

Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) PE.
Flow Cytometry: E-Cadherin Antibody (67A4) - BSA Free [NBP1-42793]

Flow Cytometry: E-Cadherin Antibody (67A4) - BSA Free [NBP1-42793]

Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) APC.

Applications for E-Cadherin Antibody (67A4) - BSA Free

Application
Recommended Usage

Flow Cytometry

5-10 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

4-8 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Flow Cytometry: Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 ug/ml per 1 million cells/ml. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: E-Cadherin

CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human)

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (67A4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for E-Cadherin Antibody (67A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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