E-Cadherin Antibody (BLR088G)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14687

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14687
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR088G

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

residues 155-707 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for E-Cadherin Antibody (BLR088G)

Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687]

Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687]

Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] . Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRPconjugated goat anti-rabbit IgG. DAB.
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Applications for E-Cadherin Antibody (BLR088G)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (BLR088G)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (BLR088G)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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