E-Cadherin Antibody (CDH1/1525)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53354
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0001.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
![Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Western-Blot-NBP2-53354-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CDH1/1525
Concentration
0.2 mg/ml
Product Summary for E-Cadherin Antibody (CDH1/1525)
Immunogen
Recombinant human E-Cadherin protein (Uniprot: P12830)
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Cell Surface
Marker
Intercellular Junction Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54551)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for E-Cadherin Antibody (CDH1/1525)
Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]
Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Immunocytochemistry-Immunofluorescence-NBP2-53354-img0007.jpg "Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]
Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0009.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0002.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0004.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
![Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-CDH1-1525-Protein-Array-NBP2-53354-img0008.jpg "Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354]")
Applications for E-Cadherin Antibody (CDH1/1525)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
UniProt
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (CDH1/1525)
Product Specific Notices for E-Cadherin Antibody (CDH1/1525)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...