Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # CDH1/4398R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to E-Cadherin residues within aa600-700 of E-cadherin (Uniprot: P12830)

Localization

Cell surface

Marker

Intercellular Junction Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-11401).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/4398R).
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082] - Formalin-fixed, paraffin-embedded human cervix stained with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/4398R).
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free [NBP3-09082] - Formalin-fixed, paraffin-embedded human cervix stained with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/4398R).
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Applications for E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT.Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-Cadherin, a member of the cadherin superfamily, is implicated as a key player in different cellular processes including development, morphology, polarity, migration and tissue integrity (1-2). E-cadherin is a glycoprotein with an extracellular domain that interacts with other E-cadherin molecules on adjacent cells, thereby establishing adhesion between epithelial cells. Established as a tumor and metastasis suppressor, E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis (3-4). Mutations in E-cadherin are found in gastric, breast, colorectal, thyroid and ovarian cancer (5).

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (CDH1/4398R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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