E-Cadherin Antibody (CL1172) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34476

Novus Biologicals, part of Bio-Techne
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NBP2-34476
NBP2-34476-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1172

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P12830, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for E-Cadherin Antibody (CL1172) - BSA Free

Western Blot: E-Cadherin Antibody (CL1172) [NBP2-34476]

Western Blot: E-Cadherin Antibody (CL1172) [NBP2-34476]

Western Blot: E-Cadherin Antibody (CL1172) [NBP2-34476] - Lane 1: Marker [kDa,Lane 2: Human cell line MCF-7, Lane 3: Human cell line SK-BR-3, Lane 4: Human cell line HeLa
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476] - Staining of human prostate shows strong membranous positivity in glandular cells.
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Applications for E-Cadherin Antibody (CL1172) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (CL1172) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (CL1172) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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