E-Cadherin Antibody (DECMA-1)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-11512

Novus Biologicals, part of Bio-Techne
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NB120-11512SS
NB120-11512
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Feline

Cited:

Mouse, Bovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Microarray, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # DECMA-1

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

Mouse embryonal carcinoma cell line PCC4 Aza RI.

Localization

Cell membrane; cell-cell junction; single-pass type I membrane protein

Specificity

Antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin

Marker

Epithelial Cell Marker, Adherens Junctions Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for E-Cadherin Antibody (DECMA-1)

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Adult mouse small intestine stained with NB12-11512. Image from verified customer review.
Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.
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Applications for E-Cadherin Antibody (DECMA-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1600

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:3000
Application Notes
The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in westernblot or immunohistochemical assays.

Reviewed Applications

Read 2 reviews rated 4 using NB120-11512 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Raw ascites

Preservative

0.9% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 282637 (Bovine)

Gene Symbol

CDH1

UniProt

P09803

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (DECMA-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (DECMA-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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