E-Cadherin Antibody (rCDH1/6769)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23653

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-23653-20ug
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rCDH1/6769

Concentration

0.2 mg/ml

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa600-700) of human E-Cadherin (exact sequence is proprietary)

Localization

Cell Surface

Marker

Intercellular Junction Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for E-Cadherin Antibody (rCDH1/6769)

E-Cadherin Antibody (rCDH1/6769) Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] - IHC analysis of formalin-fixed, paraffin-embedded human stomach. Stained using E-Cadherin Antibody (rCDH1/6769) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
E-Cadherin Antibody (rCDH1/6769) Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] - Formalin-fixed, paraffin-embedded human colon stained with E-Cadherin Antibody (rCDH1/6769). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for E-Cadherin Antibody (rCDH1/6769)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (rCDH1/6769)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (rCDH1/6769)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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