E-Tag Epitope Tag Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-518
![Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-518]](https://resources.bio-techne.com/images/products/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-518-img0004.jpg "Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-518]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Goats were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for E-Tag Epitope Tag Antibody - BSA Free
Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-518]
Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for E-Tag Epitope Tag Antibody - BSA Free
Application
Recommended Usage
ELISA
Coating-1:100-1:500 Primary-1:1000-1:30000
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Immunoprecipitation
1 - 4 ug/mg lysate
Western Blot
1:1000 - 1:30000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E-Tag Epitope Tag
Alternate Names
E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag
Additional E-Tag Epitope Tag Products
Product Documents for E-Tag Epitope Tag Antibody - BSA Free
Product Specific Notices for E-Tag Epitope Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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