E-Tag Epitope Tag Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-527
![Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]](https://resources.bio-techne.com/images/products/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0004.jpg "Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Rabbits were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E-Tag Epitope Tag Antibody - BSA Free
Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]
Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - Analysis using the HRP conjugate of NB600-527. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).![Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]](https://resources.bio-techne.com/images/products/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0002.jpg "Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]")
Western Blot: E-Tag Epitope Tag AntibodyBSA Free [NB600-527]
Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).Applications for E-Tag Epitope Tag Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000- 1:30000
Immunocytochemistry/ Immunofluorescence
1:100-1:400
Immunoprecipitation
1-4 ug/mg lysate
Western Blot
1:1000 - 1:30000
Application Notes
ELISA - Coating: 1:100-1:500.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E-Tag Epitope Tag
Alternate Names
E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag
Additional E-Tag Epitope Tag Products
Product Documents for E-Tag Epitope Tag Antibody - BSA Free
Product Specific Notices for E-Tag Epitope Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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