E-Tag Epitope Tag Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05892
![Western Blot: E-Tag Epitope Tag Antibody [NBP3-05892]](https://resources.bio-techne.com/images/products/E-Tag-Epitope-Tag-Antibody-Western-Blot-NBP3-05892-img0003.jpg "Western Blot: E-Tag Epitope Tag Antibody [NBP3-05892]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for E-Tag Epitope Tag Antibody
Immunogen
Produced in rabbits immunized with A synthetic peptide corresponding to the E-Tag Epitope Tag sequence (GAPVPYPDPLEPR).
Specificity
Recognizes N-terminal and C-terminal E tag in fusion proteins
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for E-Tag Epitope Tag Antibody
Western Blot: E-Tag Epitope Tag Antibody [NBP3-05892]
Western Blot: E-Tag Epitope Tag Antibody [NBP3-05892] - A: Etag-GFERh(C) transfected 293 cell lysate at 2ugLane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ugLane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ugLane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ugLane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ugLane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ugLane G: Nagetive control at 10ugSecondaryDylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.![Immunocytochemistry/ Immunofluorescence: E-Tag Epitope Tag Antibody [NBP3-05892]](https://resources.bio-techne.com/images/products/E-Tag-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05892-img0001.jpg "Immunocytochemistry/ Immunofluorescence: E-Tag Epitope Tag Antibody [NBP3-05892]")
Immunocytochemistry/ Immunofluorescence: E-Tag Epitope Tag Antibody [NBP3-05892]
Immunocytochemistry/Immunofluorescence: E-Tag Epitope Tag Antibody [NBP3-05892] - Staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-E-Tag polyclonal antibody at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).![Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-05892]](https://resources.bio-techne.com/images/products/E-Tag-Epitope-Tag-Antibody-Immunoprecipitation-NBP3-05892-img0002.jpg "Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-05892]")
Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-05892]
Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-05892] - Lane A:0.5 mg E tag-1M-125D-His Whole Cell LysateLane B:0.5 mg His-E tag-1M-125D Whole Cell LysateLane C:0.5 mg His-1M-125D-E tag Whole Cell LysateLane D:0.5 mg E tag-1M-244P-His Whole Cell LysateLane E:0.5 mg Psetp2 Whole Cell Lysate2 uL anti-E-tag rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-E-tag rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Applications for E-Tag Epitope Tag Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Immunoprecipitation
0.5-2 ug/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E-Tag Epitope Tag
Alternate Names
E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag
Additional E-Tag Epitope Tag Products
Product Documents for E-Tag Epitope Tag Antibody
Product Specific Notices for E-Tag Epitope Tag Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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