E2F-4 Antibody (5B7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00001874-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B7

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all E2F-4 Antibodies »

Product Specifications

Immunogen

E2F4 (NP_001941, 211 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQ

Specificity

E2F4 - E2F transcription factor 4, p107/p130-binding

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for E2F-4 Antibody (5B7) - Azide and BSA Free

Western Blot: E2F-4 Antibody (5B7) [H00001874-M01]

Western Blot: E2F-4 Antibody (5B7) [H00001874-M01]

Western Blot: E2F-4 Antibody (5B7) [H00001874-M01] - E2F4 monoclonal antibody (M01), clone 5B7 Analysis of E2F4 expression in A-549.
Sandwich ELISA: E2F-4 Antibody (5B7) [H00001874-M01] - Detection limit for recombinant GST tagged E2F4 is approximately 0.03ng/ml as a capture antibody.

Applications for E2F-4 Antibody (5B7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E2F-4

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Long Name

E2F Transcription Factor 4

Alternate Names

E2F4

Entrez Gene IDs

1874 (Human)

Gene Symbol

E2F4

OMIM

600659 (Human)

UniProt

NP_001941 (Human)

Additional E2F-4 Products

Product Documents for E2F-4 Antibody (5B7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for E2F-4 Antibody (5B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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