E2F-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98787
![Western Blot: E2F-4 Antibody [NBP2-98787]](https://resources.bio-techne.com/images/products/E2F-4-Antibody-Western-Blot-NBP2-98787-img0002.jpg "Western Blot: E2F-4 Antibody [NBP2-98787]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human E2F-4.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for E2F-4 Antibody
Western Blot: E2F-4 Antibody [NBP2-98787]
Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa![Immunocytochemistry/ Immunofluorescence: E2F-4 Antibody [NBP2-98787]](https://resources.bio-techne.com/images/products/E2F-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98787-img0001.jpg "Immunocytochemistry/ Immunofluorescence: E2F-4 Antibody [NBP2-98787]")
Immunocytochemistry/ Immunofluorescence: E2F-4 Antibody [NBP2-98787]
Immunocytochemistry/Immunofluorescence: E2F-4 Antibody [NBP2-98787] - Immunofluorescence staining of E2F-4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human E2F-4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.Applications for E2F-4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E2F-4
Long Name
E2F Transcription Factor 4
Alternate Names
E2F4
Gene Symbol
E2F4
Additional E2F-4 Products
Product Specific Notices for E2F-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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