E2F3 Antibody (5F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001871-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all E2F3 Antibodies »

Product Specifications

Immunogen

E2F3 (NP_001940, 336 a.a. ~ 425 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QIHLASTQGPIEVYLCPEETETHSPMKTNNQDHNGNIPKPASKDLASTNSGHSDCSVSMGNLSPLASPANLLQQTEDQIPSNLEGPFVNL

Specificity

E2F3 - E2F transcription factor 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for E2F3 Antibody (5F7)

Western Blot: E2F3 Antibody (5F7) [H00001871-M01]

Western Blot: E2F3 Antibody (5F7) [H00001871-M01]

Western Blot: E2F3 Antibody (5F7) [H00001871-M01] - E2F3 monoclonal antibody (M01), clone 5F7 Analysis of E2F3 expression in COLO 320 HSR.
Proximity Ligation Assay: E2F3 Antibody (5F7) [H00001871-M01]

Proximity Ligation Assay: E2F3 Antibody (5F7) [H00001871-M01]

Proximity Ligation Assay: E2F3 Antibody (5F7) [H00001871-M01] - Analysis of protein-protein interactions between MSH2 and E2F3. HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-E2F3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: E2F3 Antibody (5F7) [H00001871-M01] - Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.

Applications for E2F3 Antibody (5F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E2F3

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

Alternate Names

DKFZp686C18211, E2F transcription factor 3, E2F-3, KIAA0075, MGC104598, transcription factor E2F3

Entrez Gene IDs

1871 (Human)

Gene Symbol

E2F3

OMIM

600427 (Human)

UniProt

NP_001940 (Human)

Additional E2F3 Products

Product Documents for E2F3 Antibody (5F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E2F3 Antibody (5F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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