E2F3 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81745CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-81745AF350

Alexa Fluor 405

NBP2-81745AF405

Alexa Fluor 488

NBP2-81745AF488

Alexa Fluor 532

NBP2-81745AF532

Alexa Fluor 594

NBP2-81745AF594

Alexa Fluor 647

NBP2-81745AF647

Alexa Fluor 700

NBP2-81745AF700

Alexa Fluor 750

NBP2-81745AF750

Biotin

NBP2-81745B

DyLight 350

NBP2-81745UV

DyLight 405

NBP2-81745V

DyLight 488

NBP2-81745G

DyLight 550

NBP2-81745R

DyLight 594

NBP2-81745DL594

DyLight 650

NBP2-81745C

DyLight 680

NBP2-81745FR

DyLight 755

NBP2-81745IR

FITC

NBP2-81745F

HRP

NBP2-81745H

Janelia Fluor 525

NBP2-81745JF525

Janelia Fluor 549

NBP2-81745JF549

Janelia Fluor 585

NBP2-81745JF585

Janelia Fluor 635

NBP2-81745JF635

Janelia Fluor 646

NBP2-81745JF646

Janelia Fluor 669

NBP2-81745JF669

mFluor Violet 450 SE

NBP2-81745MFV450

mFluor Violet 500 SE

NBP2-81745MFV500

mFluor Violet 610 SE

NBP2-81745MFV610

PerCP

NBP2-81745PCP

Forumulation

Catalog #

BSA Free

NBP2-81745

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E2F3 Antibodies »

Product Specifications

Immunogen

E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3. Amino Acid Squence: PDSPKTPKSPSEKT

Specificity

E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for E2F3 Antibody [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: E2F3

E2F3 is encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

Alternate Names

DKFZp686C18211, E2F transcription factor 3, E2F-3, KIAA0075, MGC104598, transcription factor E2F3

Gene Symbol

E2F3

Additional E2F3 Products

Product Documents for E2F3 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for E2F3 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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