E2F8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52650
![Western Blot: E2F8 Antibody [NBP1-52650]](https://resources.bio-techne.com/images/products/E2F8-Antibody-Western-Blot-NBP1-52650-img0006.jpg "Western Blot: E2F8 Antibody [NBP1-52650]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 817 and 867 of human E2F Transcription Factor 8 using the numbering given in entry NP_078956.2 (GeneID 79733).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E2F8 Antibody
Western Blot: E2F8 Antibody [NBP1-52650]
Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: E2F8 Antibody [NBP1-52650]](https://resources.bio-techne.com/images/products/E2F8-Antibody-Immunoprecipitation-NBP1-52650-img0007.jpg "Immunoprecipitation: E2F8 Antibody [NBP1-52650]")
Immunoprecipitation: E2F8 Antibody [NBP1-52650]
Immunoprecipitation: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody NBP1-52650(lot NBP1-52650-3) used for IP at 6 ug per reaction. E2F8 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-52650-2). For blotting immunoprecipitated E2F8, NBP1-52650 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for E2F8 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: E2F8
Alternate Names
E2F family member 8, E2F transcription factor 8, E2F-8, FLJ23311, transcription factor E2F8
Gene Symbol
E2F8
Additional E2F8 Products
Product Documents for E2F8 Antibody
Product Specific Notices for E2F8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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