E6AP/UBE3A Antibody (2F6)

Name: E6AP/UBE3A Antibody (2F6)
Brand: Novus Biologicals
SKU: H00007337-M01

Novus Biologicals, part of Bio-Techne | Catalog # H00007337-M01

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Citations (4)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

UBE3A (AAH09271, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCR

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33737669).

Specificity

UBE3A - ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for E6AP/UBE3A Antibody (2F6)

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.

Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A expression in Jurkat ( Cat # L017V1 ).

ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Detection limit for recombinant GST tagged UBE3A is approximately 0.03ng/ml as a capture antibody.

Knockdown Validated: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Knockdown Validated: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - E6AP knockdown was confirmed by immunoblotting. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02483/full), licensed under a CC-BY license.

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] -

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Effect of E6AP silencing on the stability of HPV E6. (A)E6AP knockdown was confirmed by immunoblotting. (B) HEK293T cells (E6AP-null) were transfected with HPV16 E6 or HPV18 E6. (C) After 48 h the cells were harvested, & protein levels analyzed by immunoblotting. beta-actin acted as a control for transfection efficiency. (D) HEK293T cells (E6AP-null) were transfected with the indicated expression plasmids; after 36 h they were treated with 10 μM MG132 & 12 h later, harvested & subjected to immunoprecipitation (IP) with anti-myc-conjugated agarose beads. Polyubiquitinated HPV E6 was then detected for IP with an antibody against HA. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31749782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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