E74 like factor 1 Antibody (3B7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001997-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3B7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all E74 like factor 1 Antibodies »

Product Specifications

Immunogen

ELF1 (NP_758961, 421 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IQAPTQVPVVVSPRNQQLHTVTLQTVPLTTVIASTDPSAGTGSQKFILQAIPSSQPMTVLKENVMLQSQKAGSPPSIVLGPAQVQQVLTSNVQTICNGTV

Specificity

ELF1 - E74-like factor 1 (ets domain transcription factor)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for E74 like factor 1 Antibody (3B7)

Western Blot: E74 like factor 1 Antibody (3B7) [H00001997-M01]

Western Blot: E74 like factor 1 Antibody (3B7) [H00001997-M01]

Western Blot: E74 like factor 1 Antibody (3B7) [H00001997-M01] - ELF1 monoclonal antibody (M01), clone 3B7 Analysis of ELF1 expression in 293.
Sandwich ELISA: E74 like factor 1 Antibody (3B7) [H00001997-M01] - Detection limit for recombinant GST tagged ELF1 is approximately 1ng/ml as a capture antibody.

Applications for E74 like factor 1 Antibody (3B7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E74 like factor 1

E74-like factor 1 (ELF1) is a member of the ETS (e26 transformation specific sequence) family of proteins, contains an ETS-domain, and shares sequence identity with the Drosophila ecdysone inducible E74 transcription factor. ETS was originally identified in the E26 retroviral isolate that led to the discovery of c-ets-1 (cellular ets-1), a cellular oncogenic transcription factor capable of transforming cells. Like ETS, ELF-1 functions as a transcription factor and has been shown to bind promoter and enhancer elements in a number of immunity responsive genes. ELF-1 is highly expressed in B-cells and appears to play an integral role in B-cell gene expression.

Alternate Names

E74-like factor 1, E74-like factor 1 (ets domain transcription factor), ETS-related transcription factor Elf-1

Entrez Gene IDs

1997 (Human)

Gene Symbol

ELF1

OMIM

189973 (Human)

UniProt

NP_758961 (Human)

Additional E74 like factor 1 Products

Product Documents for E74 like factor 1 Antibody (3B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E74 like factor 1 Antibody (3B7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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