E74 like factor 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1069
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-AMKQNELLEPNSF corresponding to C-Terminus according to NP_758961.1, NP_001138825.1.
Localization
Nuclear
Specificity
This antibody is expected to recognise both reported isoforms.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for E74 like factor 1 Antibody
![Western Blot: E74 like factor 1 Antibody [NB100-1069]](https://resources.bio-techne.com/images/products/E74-like-factor-1-Antibody-Western-Blot-NB100-1069-img0005.jpg "Western Blot: E74 like factor 1 Antibody [NB100-1069]")
Western Blot: E74 like factor 1 Antibody [NB100-1069]
Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.3ug/ml) staining of Human Lymph Node (A), Human Tonsil (B) and Mouse Thymus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069]](https://resources.bio-techne.com/images/products/E74-like-factor-1-Antibody-Immunohistochemistry-Paraffin-NB100-1069-img0006.jpg "Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069]")
Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069]
Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Western Blot: E74 like factor 1 Antibody [NB100-1069]](https://resources.bio-techne.com/images/products/E74-like-factor-1-Antibody-Western-Blot-NB100-1069-img0004.jpg "Western Blot: E74 like factor 1 Antibody [NB100-1069]")
Western Blot: E74 like factor 1 Antibody [NB100-1069]
Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for E74 like factor 1 Antibody
Application
Recommended Usage
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
Approx 65kDa band observed in Human Lymph Node and Tonsil and in Mouse Thymus lysates (calculated MW of 64.9kDa according to Human NP_001138825.1 and 62.3kDa according to Mouse NP_001273341.1).
Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 67.5kDa according to NP_758961.1).
IHC: Paraffin embedded Human Thymus.
Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 67.5kDa according to NP_758961.1).
IHC: Paraffin embedded Human Thymus.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: E74 like factor 1
Alternate Names
E74-like factor 1, E74-like factor 1 (ets domain transcription factor), ETS-related transcription factor Elf-1
Gene Symbol
ELF1
UniProt
Additional E74 like factor 1 Products
Product Documents for E74 like factor 1 Antibody
Product Specific Notices for E74 like factor 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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