EAAT2/GLT1 Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20136
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Human, Mouse, Rat
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In vivo assay, In-situ Hybridization, Western Blot
Cited:
Cytometric Bead Assay Standard, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In Situ Hybridization, In vivo assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from mouse EAAT2/GLT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
Reactivity Notes
Marmoset
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EAAT2/GLT1 Antibody - Azide Free
Western Blot: EAAT2/GLT1 AntibodyAzide Free [NBP1-20136]
Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.Immunocytochemistry/ Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136]
Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY license.Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136]
Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Hippocampal GLT-1 and NR1 expression 3 days after SE with MyD88 inhibition. Sections from the hippocampi of mice in the CP group (A1-A3) and MIP group (B1-B3) 3 days after SE with GLT-1 immunoreactivity in astrocytes and neuronal processes. (A4, B4) Higher magnification of the boxes in (A3) and (B3). Scale bars: (A1-A3, B1-B3) 100 um; (A4, B4) 50 um. SE = status epilepticus, DG=dentate gyrus, hippocampal regions CA3 and CA1, CP = control peptide, MIP = MyD88 inhibitory peptide. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30112701/) licensed under a CC-BY license.Applications for EAAT2/GLT1 Antibody - Azide Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Application Notes
Although not tested this antibody may work in IHC-Frozen. Use in flow was reported in scientific literature (PMID: 23793269). Use in In-vivo and in ICC/IF reported in scientific literature (PMID 25581361). Use in In-situ Hybridization reported in scientific literature (PMID: 26150391).
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Format
Azide Free
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EAAT2/GLT1
Long Name
Excitatory Amino Acid Transporter 2
Alternate Names
EAAT2, GLT1, SLC1A2
Gene Symbol
SLC1A2
UniProt
Additional EAAT2/GLT1 Products
Product Documents for EAAT2/GLT1 Antibody - Azide Free
Product Specific Notices for EAAT2/GLT1 Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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