EAAT2/GLT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13348

Novus Biologicals, part of Bio-Techne
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NBP3-13348
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Feline (90%), Rhesus Macaque (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EAAT2/GLT1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT2/GLT1. The exact sequence is proprietary.

Reactivity Notes

Human (88%), Dog (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for EAAT2/GLT1 Antibody

Western Blot: EAAT2/GLT1 Antibody [NBP3-13348]

Western Blot: EAAT2/GLT1 Antibody [NBP3-13348]

Western Blot: EAAT2/GLT1 Antibody [NBP3-13348] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348] - EAAT2/GLT1 antibody detects EAAT2/GLT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. EAAT2/GLT1 stained by EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348] - EAAT2/GLT1 antibody detects EAAT2/GLT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: EAAT2/GLT1 stained by EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for EAAT2/GLT1 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EAAT2/GLT1

GLT1 is the major glial glutamate transporter that takes up more than 90% of glutamate from the synapse. GLT1b is a splice variant that contains 11 unique amino acids at the C-terminus that encode a PDZ binding domain. GLT1b was initially believed to be the neuronal isoform of GLT1, but it now appears that GLT1b is localized to astrocytes in the normal brain and to neurons in pathological conditions. In Lou Gehrig's Disease, GLT1a is decreased, while GLT1b is increased. There are also different expressions in hypoxic cells, which have not yet been fully elucidated.

Long Name

Excitatory Amino Acid Transporter 2

Alternate Names

EAAT2, GLT1, SLC1A2

Gene Symbol

SLC1A2

Additional EAAT2/GLT1 Products

Product Documents for EAAT2/GLT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EAAT2/GLT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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