EAAT3 Antibody (S180-41)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59319
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S180-41
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for EAAT3 Antibody (S180-41)
Immunogen
Fusion protein amino acids 1-524 (full length) of human EAAT3 (Uniprot: P43005)
Localization
Membrane
Specificity
Detects 60kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for EAAT3 Antibody (S180-41)
Mouse Monoclonal EAAT3 Antibody (S180-41)
Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 (NBP2-59319). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (NBP2-59319) at 1:1000.
Mouse Monoclonal EAAT3 Antibody (S180-41)
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 (NBP2-59319). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (NBP2-59319) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) EAAC1 Antibody (D) Composite.
Mouse Monoclonal EAAT3 Antibody (S180-41)
Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane . Magnification: 60X.Applications for EAAT3 Antibody (S180-41)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: EAAT3
Long Name
Excitatory amino acid transporter 3
Alternate Names
EAAC1, SLC1A1
Gene Symbol
SLC1A1
Additional EAAT3 Products
Product Documents for EAAT3 Antibody (S180-41)
Product Specific Notices for EAAT3 Antibody (S180-41)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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