EB3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84924

Novus Biologicals, part of Bio-Techne
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NBP1-84924
NBP1-84924-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EB3 Antibodies »

Product Summary for EB3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKERDFYFSKLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EB3 Antibody

Western Blot: EB3 Antibody [NBP1-84924]

Western Blot: EB3 Antibody [NBP1-84924]

Western Blot: EB3 Antibody [NBP1-84924] - Analysis in human cell line U-87 MG.
Immunohistochemistry-Paraffin: EB3 Antibody [NBP1-84924]

Immunohistochemistry-Paraffin: EB3 Antibody [NBP1-84924]

Immunohistochemistry-Paraffin: EB3 Antibody [NBP1-84924] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for EB3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EB3

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Alternate Names

EB1 protein family member 3, EB3EBF3-S, End-binding protein 3, microtubule-associated protein RP/EB family member 3, microtubule-associated protein, RP/EB family, member 3, RP3EBF3APC binding protein

Gene Symbol

MAPRE3

Additional EB3 Products

Product Documents for EB3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EB3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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