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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rEBAG9/7264

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human EBAG9/RCAS1 protein

Localization

Golgi apparatus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Positive Controls: MCF-7, Jurkat or HEK-293 cell lysates. Human skeletal muscle tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for EBAG9/RCAS1 Antibody (rEBAG9/7264) - Azide and BSA Free

EBAG9/RCAS1 Antibody (rEBAG9/7264) - Azide and BSA Free

EBAG9/RCAS1 Antibody (rEBAG9/7264)

Formalin-fixed, paraffin-embedded human brain stained with EBAG9/RCAS1 antibody (rEBAG9/7264). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for EBAG9/RCAS1 Antibody (rEBAG9/7264) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: EBAG9/RCAS1

EBAG9 was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Alternate Names

Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells

Gene Symbol

EBAG9

Additional EBAG9/RCAS1 Products

Product Documents for EBAG9/RCAS1 Antibody (rEBAG9/7264) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EBAG9/RCAS1 Antibody (rEBAG9/7264) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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