Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 5P10D3

Concentration

1.0 mg/ml

View all EBI3 Antibodies »

Product Specifications

Immunogen

Full-length recombinant mouse EBI3 protein

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for EBI3 Antibody (5P10D3)

Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943]

Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943]

Western Blot: EBI3 Antibody (5P10D3) [NBP2-03943] - Analysis using EBI3 antibody. Mouse EBI recombinant protein (A) and lysate from mouse kidney (B) probed with EBI3 antibody at 0.5 and 5 ug/ml, respectively. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: EBI3 Antibody (5P10D3) [NBP2-03943]

Flow Cytometry: EBI3 Antibody (5P10D3) [NBP2-03943]

Flow Cytometry: EBI3 Antibody (5P10D3) [NBP2-03943] - Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 2 ug/10^6 cells.

Applications for EBI3 Antibody (5P10D3)

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 30660991)

Flow Cytometry

1-3ug/10^6cells

Western Blot

0.5-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008).

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

UniProt

O35228 (Mouse)

Additional EBI3 Products

Product Documents for EBI3 Antibody (5P10D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EBI3 Antibody (5P10D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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