EBI3 Antibody (9J1H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15735

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15735-100ul
NBP3-15735-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9J1H4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EBI3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 130-229 of human EBI3 (Q14213). DPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EBI3 Antibody (9J1H4)

Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735]

Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735]

Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of MCF7 cells, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [NBP3-15735]

Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [NBP3-15735]

Immunocytochemistry/Immunofluorescence: EBI3 Antibody (9J1H4) [NBP3-15735] - Immunofluorescence analysis of mouse spleen cells using EBI3 Rabbit mAb (NBP3-15735) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735]

Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735]

Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of various cell lines, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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Applications for EBI3 Antibody (9J1H4)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EBI3

EBI3 gene encodes a 229 amino acid long, 25 kDA interleukin-27 subunit beta protein that is member to the hematopoitetin receptor family. These proteins obtain pro as well as anti-inflammatory properties and have roles in T-helper cell development, suppressing cell proliferation, encouraging cytotoxic T-cell activity, and can induce isotope switching in B cells. EBI3 participates in Th1 differentiation, IL-2 signaling (and its primary biological effects in various immune cells), as well as IL27-mediated signaling events. EBI3 interacts with genes IL27, IL12A, CANX, MDF1, and SMAD3. It has been associated and researched in sepsis, asthma, carcinoma, Chron's disease, b-cell lymphomas, ulcerative colitis, Hodgkin disease, lymphoma, ebv infections, and glomerulonephritis.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

Additional EBI3 Products

Product Documents for EBI3 Antibody (9J1H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EBI3 Antibody (9J1H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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