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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Virus

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LMPO24

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified plasma membranes from EBV Latent Membrane Protein 1 expressing insect cells infected with a recombinant EBV Latent Membrane Protein 1 baculovirus

Specificity

Niedobitek et al., 1995 (PMID: 7605996): "The LMPO24 clone demonstrates strong and specific reactivity in immunohistochemical staining of paraffin-embedded tissues."

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free

Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free [NBP2-50383]

Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free [NBP2-50383]

Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) [NBP2-50383] - Intracellular staining of LMP1 using LMPO24 antibody. NK and T cell lines were fixed and permeabilised with an EBiosciences kit (Cat 88-8824-00) then stained with LMPO24 monoclonal antibody, followed by detection with Alexa Fluor 488 conjugated anti-mouse IgG antibody and analysis by flow cytometry. The histograms show negative control fluorescence staining (black lines) and LMPO24 staining (red lines). MEC04, SNK1, SNK6 and SNT8 are EBV positive tumour cell lines derived from patients with nasal NK/T cell lymphomas. MEC04, SNK1 and SNK6 are NK cell lines, while SNT8 is a T cell line. MEC04 lacks LMP1 expression, SNK1 expresses weak to moderate levels of LMP1, whilst SNK6 and SNT8 express high levels of LMP1.

Applications for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1:10 - 1:1000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500
Application Notes
Positive control(s): EBV transformed lymphoblastoid cell lines. This antibody is CyTOF-ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBV Latent Membrane Protein 1

Additional EBV Latent Membrane Protein 1 Products

Product Documents for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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