ECH1 Antibody (002)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90288

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 002

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ECH1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ECH1 (Uniprot#: Q13011; Thr34-Leu328).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ECH1 Antibody (002)

Western Blot: ECH1 Antibody (002) [NBP2-90288]

Western Blot: ECH1 Antibody (002) [NBP2-90288]

Western Blot: ECH1 Antibody (002) [NBP2-90288] - Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDaObserved band size:36 kDa.

Applications for ECH1 Antibody (002)

Application
Recommended Usage

ELISA

1:5000-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECH1

ECH1 encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Alternate Names

delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, delta3,5-delta2,4-dienoyl-CoA isomerase, dienoyl-CoA isomerase, EC 5.3.3.-, enoyl CoA hydratase 1, peroxisomal, enoyl Coenzyme A hydratase 1, peroxisomal, HPXEL, peroxisomal enoyl-CoA hydratase 1

Gene Symbol

ECH1

Additional ECH1 Products

Product Documents for ECH1 Antibody (002)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ECH1 Antibody (002)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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