ECH1 Antibody (5G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00001891-M01

Novus Biologicals, part of Bio-Techne
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H00001891-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ECH1 Antibodies »

Product Specifications

Immunogen

ECH1 (NP_001389, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSNYPGLSISLRLTGSSAQEEASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDL

Specificity

ECH1 - enoyl Coenzyme A hydratase 1, peroxisomal

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ECH1 Antibody (5G8)

Western Blot: ECH1 Antibody (5G8) [H00001891-M01]

Western Blot: ECH1 Antibody (5G8) [H00001891-M01]

Western Blot: ECH1 Antibody (5G8) [H00001891-M01] - Analysis of ECH1 expression in transfected 293T cell line by ECH1 monoclonal antibody (M01), clone 5G8.Lane 1: ECH1 transfected lysate(35.8 KDa).Lane 2: Non-transfected lysate.

Applications for ECH1 Antibody (5G8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ECH1

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Alternate Names

delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, delta3,5-delta2,4-dienoyl-CoA isomerase, dienoyl-CoA isomerase, EC 5.3.3.-, enoyl CoA hydratase 1, peroxisomal, enoyl Coenzyme A hydratase 1, peroxisomal, HPXEL, peroxisomal enoyl-CoA hydratase 1

Entrez Gene IDs

1891 (Human)

Gene Symbol

ECH1

OMIM

600696 (Human)

UniProt

NP_001389 (Human)

Additional ECH1 Products

Product Documents for ECH1 Antibody (5G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ECH1 Antibody (5G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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