ECHDC1 Antibody

Western Blot: ECHDC1 Antibody [NBP3-12637]

Western Blot: ECHDC1 Antibody [NBP3-12637] - Lane A: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)

Immunocytochemistry/ Immunofluorescence: ECHDC1 Antibody [NBP3-12637]

Immunocytochemistry/Immunofluorescence: ECHDC1 Antibody [NBP3-12637] - Staining of ECHDC1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ECHDC1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: ECHDC1 Antibody [NBP3-12637]

Immunoprecipitation: ECHDC1 Antibody [NBP3-12637] - Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-ECHDC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ECHDC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa