ECHDC3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82159
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ECHDC3 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FTGEPISAQEALLHGLLSKVVPEAELQEETMRIARKIASLSRPVVSLGKATFYKQLPQDLGTAYYLTSQAMVDNLALRDGQEGITAFLQKRKPVWSH
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (81%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ECHDC3 Antibody
Immunocytochemistry/ Immunofluorescence: ECHDC3 Antibody [NBP1-82159]
Immunocytochemistry/Immunofluorescence: ECHDC3 Antibody [NBP1-82159] - Staining of human cell line A-431 shows positivity in nucleus, nucleoli and mitochondria. Antibody stainng is shown in green.Immunohistochemistry-Paraffin: ECHDC3 Antibody [NBP1-82159]
Immunohistochemistry-Paraffin: ECHDC3 Antibody [NBP1-82159] - Staining of human cerebral cortex shows low expression as expected.Applications for ECHDC3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ECHDC3
Alternate Names
enoyl CoA hydratase domain containing 3, enoyl Coenzyme A hydratase domain containing 3, FLJ20909, mitochondrial
Gene Symbol
ECHDC3
Additional ECHDC3 Products
Product Documents for ECHDC3 Antibody
Product Specific Notices for ECHDC3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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