ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34747
![Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free [NBP2-34747]](https://resources.bio-techne.com/images/products/ECM-1-Secretory-Component-P85-Antibody-SPM217-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34747-img0002.jpg "Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free [NBP2-34747]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM217
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Secretory Component protein isolated from human colostrum (Uniprot: Q16610 )
Localization
Cell surface and cytoplasmic
Specificity
This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32869).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free [NBP2-34747]
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free [NBP2-34747] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with IgA secretory component Monoclonal Antibody (SPM217).Applications for ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ECM1
Long Name
Extracellular Matrix Protein 1
Alternate Names
ECM1, p85, Secretory Component P85
Gene Symbol
ECM1
Additional ECM1 Products
Product Documents for ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free
Product Specific Notices for ECM-1/Secretory Component P85 Antibody (SPM217) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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