ECSIT Antibody (1B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00051295-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ECSIT Antibodies »

Product Specifications

Immunogen

ECSIT (AAH00193, 20 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Reactivity Notes

Human. Other species not tested.

Specificity

SITPEC - signaling intermediate in Toll pathway, evolutionarily conserved

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ECSIT Antibody (1B8)

Proximity Ligation Assay: ECSIT Antibody (1B8) [H00051295-M01]

Proximity Ligation Assay: ECSIT Antibody (1B8) [H00051295-M01]

Proximity Ligation Assay: ECSIT Antibody (1B8) [H00051295-M01] - Analysis of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal 1:1200 and anti-ECSIT mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for ECSIT Antibody (1B8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein on ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ECSIT

Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Alternate Names

ECSIT homolog (Drosophila), evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved, Protein SITPEC, signaling intermediate in Toll pathway evolutionarily conserved ortholog, SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Entrez Gene IDs

51295 (Human)

Gene Symbol

ECSIT

OMIM

608388 (Human)

UniProt

AAH00193 (Human)

Additional ECSIT Products

Product Documents for ECSIT Antibody (1B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ECSIT Antibody (1B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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