EDF1 Antibody (3E6)

Novus Biologicals, part of Bio-Techne | Catalog # H00008721-M03

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EDF1 Antibodies »

Product Specifications

Immunogen

EDF1 (AAH15500.1, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQSRGEDVETSKKWAAGQNKQHSITKNTAKLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIEKGPRAK

Specificity

Reacts with endothelial differentiation-related factor 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EDF1 Antibody (3E6)

Western Blot: EDF1 Antibody (3E6) [H00008721-M03]

Western Blot: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of EDF1 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/ Immunofluorescence: EDF1 Antibody (3E6) [H00008721-M03]

Immunocytochemistry/Immunofluorescence: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of monoclonal antibody to EDF1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: EDF1 Antibody (3E6) [H00008721-M03]

Immunohistochemistry-Paraffin: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of monoclonal antibody to EDF1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml

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Applications for EDF1 Antibody (3E6)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EDF1

This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. [provided by RefSeq]

Alternate Names

EDF-1Multiprotein-bridging factor 1, endothelial differentiation-related factor 1, MBF1, MGC9058, multiprotein bridging factor 1, multiprotein bridging factor-1

Entrez Gene IDs

8721 (Human)

Gene Symbol

EDF1

UniProt

AAH15500 (Human)

Additional EDF1 Products

Product Documents for EDF1 Antibody (3E6)

Product Datasheets

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COA

SDS

Product Specific Notices for EDF1 Antibody (3E6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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