EDF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84011

Novus Biologicals, part of Bio-Techne
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NBP1-84011
NBP1-84011-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EDF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EDF1 Antibody

Western Blot: EDF1 Antibody [NBP1-84011]

Western Blot: EDF1 Antibody [NBP1-84011]

Western Blot: EDF1 Antibody [NBP1-84011] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: EDF1 Antibody [NBP1-84011]

Immunocytochemistry/ Immunofluorescence: EDF1 Antibody [NBP1-84011]

Immunocytochemistry/Immunofluorescence: EDF1 Antibody [NBP1-84011] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011]

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human lymph node showing strong nuclear and cytoplasmic postitivity in geminal and non-geminal center cells.
View 3 more images

Applications for EDF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EDF1

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Alternate Names

EDF-1Multiprotein-bridging factor 1, endothelial differentiation-related factor 1, MBF1, MGC9058, multiprotein bridging factor 1, multiprotein bridging factor-1

Gene Symbol

EDF1

Additional EDF1 Products

Product Documents for EDF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EDF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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