Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all EDF1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 98 to 148 of human Endothelial Differentiation-Related Factor 1 using the numbering given in entry NP_003783.1 (GeneID 8721).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EDF1 Antibody

Western Blot: EDF1 Antibody [NBP2-22327]

Western Blot: EDF1 Antibody [NBP2-22327]

Western Blot: EDF1 Antibody [NBP2-22327] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22327 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: EDF1 Antibody [NBP2-22327]

Immunohistochemistry: EDF1 Antibody [NBP2-22327]

Immunohistochemistry: EDF1 Antibody [NBP2-22327] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- EDF-1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: EDF1 Antibody [NBP2-22327]

Immunoprecipitation: EDF1 Antibody [NBP2-22327]

Immunoprecipitation: EDF1 Antibody [NBP2-22327] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22327 used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody. For blotting immunoprecipitated EDF-1, NBP2-22327 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for EDF1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EDF1

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Alternate Names

EDF-1Multiprotein-bridging factor 1, endothelial differentiation-related factor 1, MBF1, MGC9058, multiprotein bridging factor 1, multiprotein bridging factor-1

Gene Symbol

EDF1

UniProt

NP_003783.1 (Human)

Additional EDF1 Products

Product Documents for EDF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EDF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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