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EDNRA/Endothelin R Type A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33614

Novus Biologicals, part of Bio-Techne
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NBP1-33614

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Bovine, Equine

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDNRA/Endothelin R Type A. The exact sequence is proprietary.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26980806).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EDNRA/Endothelin R Type A Antibody

Western Blot: EDNRA/Endothelin R Type A Antibody [NBP1-33614]

Western Blot: EDNRA/Endothelin R Type A Antibody [NBP1-33614]

Western Blot: EDNRA/Endothelin R Type A Antibody [NBP1-33614] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin A Receptor antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: EDNRA/Endothelin R Type A Antibody [NBP1-33614]

Immunohistochemistry-Paraffin: EDNRA/Endothelin R Type A Antibody [NBP1-33614]

Immunohistochemistry-Paraffin: EDNRA/Endothelin R Type A Antibody [NBP1-33614] - SW480 xenograft , using Endothelin A Receptor antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: EDNRA/Endothelin R Type A Antibody [NBP1-33614]

Western Blot: EDNRA/Endothelin R Type A Antibody [NBP1-33614]

Western Blot: EDNRA/Endothelin R Type A Antibody [NBP1-33614] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin A Receptor antibody [C3], C-term diluted at 1:0. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for EDNRA/Endothelin R Type A Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EDNRA/Endothelin R Type A

Endothelin A receptor binds endothelins, induces intracellular calcium flux and arachidonic acid accumulation. It is a member of the G protein coupled receptor family. Endothelin A receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells.

Long Name

Endothelin Receptor Type A

Alternate Names

EDNRA, ETAR, ETRA

Entrez Gene IDs

1909 (Human); 13617 (Mouse)

Gene Symbol

EDNRA

UniProt

Additional EDNRA/Endothelin R Type A Products

Product Documents for EDNRA/Endothelin R Type A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EDNRA/Endothelin R Type A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.