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EDNRB/Endothelin R Type B Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30599

Novus Biologicals, part of Bio-Techne
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NBP1-30599SS
NBP1-30599

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

A synthetic peptide from the c-terminal of human EDNRB/Endothelin R Type B conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for EDNRB/Endothelin R Type B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml
Application Notes
WB. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user. Although not confirmed, this antibody may be useful in IHC-Paraffin/Frozen. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25572612)
The sample size is shipped in liquid form.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Sterilized water

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EDNRB/Endothelin R Type B

Endothelin receptor type B (ETRB) is a G protein-coupled receptor that activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. A splice variant (SVR), differing in the intracellular C-terminal domain, has been described for this receptor. Both WT and the variant bind ET1. However, they exhibit different responses upon binding suggesting that these two variants are functionally distinct.

Long Name

Endothelin Receptor Type B

Alternate Names

EDNRB, ET-B, ETRB

Entrez Gene IDs

1910 (Human); 13618 (Mouse); 50672 (Rat)

Gene Symbol

EDNRB

Additional EDNRB/Endothelin R Type B Products

Product Documents for EDNRB/Endothelin R Type B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EDNRB/Endothelin R Type B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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